稀土含量对Ca-P陶瓷涂层组织及细胞相容性的影响

doi:10.11933/j.issn.1007-9289.2016.05.008

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中国表面工程 ›› 2016, Vol. 29 ›› Issue (5) : 66-71. DOI: 10.11933/j.issn.1007-9289.2016.05.008

表面工程

稀土含量对Ca-P陶瓷涂层组织及细胞相容性的影响

白杨,刘其斌,徐鹏,朱益志,张时维

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Effects of Rare Earth Content on Microstructure and Cell Compatibility of Ca-P Ceramic Coating Fabricated by Laser Cladding

BAI Yang, LIU Qi-bin, XU Peng, ZHU Yi-zhi and ZHANG Shi-wei

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摘要

利用激光熔覆技术在医用钛合金表面制备一层梯度Ca-P陶瓷涂层，并研究了La₂O₃含量对其微观组织结构及细胞相容性的影响。通过OM，XRD，SEM，MTT法及倒置荧光显微镜对Ca-P陶瓷涂层的结合界面、物相、表面形貌，细胞活性及细胞生长形态进行分析。结果表明：Ca-P陶瓷涂层主要含有CaTiO₃、HA、TiO₂、β-TCP等物相。当Ca-P陶瓷涂层中La₂O₃质量分数为0.6%时，在2θ为32~33°附近其XRD图谱中羟基磷灰石（HA）的特征峰较高，表明其生成的生物活性相最多。并且其在模拟体液（SBF）浸泡14天后的表面上生成的HA最多，分布最广。细胞试验表明当La₂O₃质量分数为0.4%~0.6%时，梯度生物陶瓷涂层具有较佳的细胞相容性，有利于细胞的稳定增殖与生长。

Abstract

Ca-P ceramic coating was prepared on the surface of medical titanium alloy by laser cladding technology. The effect of La₂O₃ content on microstructure and cell compatibility was studied. The bonding inerface, phase, surface morphology, bioactivity and cell growth morphology of Ca-P ceramic coating were characterized by OM, XRD, SEM, MTT assay and inverted fluorescence microscope. The results indicate that Ca-P ceramic coating mainly contains CaTiO₃, HA, TiO₂ and β-TCP. When the content of La₂O₃ added in the ceramic coating is 0.6%, the peak of hydroxyapatite (HA) reaches the maximum diffraction intensity at 2θ=32-33° in the XRD patterns, indicating that most biologically active phases are synthesized. The HA on the Ca-P ceramic coating immersed in simulated body fluid (SBF) for 14 days is maximum and extensively distributed. Cell experiments show that when the content of La₂O₃ is 0.4%-0.6%, Ca-P ceramic coating has better cell compatibility and is conducive to the stable proliferation and growth of cells.

导出引用

白杨,刘其斌,徐鹏,朱益志,张时维. 稀土含量对Ca-P陶瓷涂层组织及细胞相容性的影响[J]. 中国表面工程, 2016, 29(5): 66-71 https://doi.org/10.11933/j.issn.1007-9289.2016.05.008

BAI Yang, LIU Qi-bin, XU Peng, ZHU Yi-zhi and ZHANG Shi-wei. Effects of Rare Earth Content on Microstructure and Cell Compatibility of Ca-P Ceramic Coating Fabricated by Laser Cladding[J]. China Surface Engineering, 2016, 29(5): 66-71 https://doi.org/10.11933/j.issn.1007-9289.2016.05.008

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国家自然科学基金（51362004）

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2023-12-19

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