| 摘要: |
| 采用层层自组装法联合溶解法在NiTi合金表面制作疏松多孔的聚丙烯酸（PAA）和聚丙烯胺盐酸盐（PAH）复合有机层，在该有机层表面进行壳聚糖/骨形态蛋白2（BMP-2）涂层处理。利用扫描电镜观察逐层处理后合金表面结构变化；用接触角检测仪检测材料表面亲水性；利用BMP-2 ELASA试剂盒检测在模拟体液中rhBMP2的释放情况；经过修饰的材料分别与小鼠成骨细胞共培养，检测不同时期培养液中碱性磷酸酶活性。结果表明：经盐酸溶蚀后PAA/PAH涂层广泛形成纳米至微米级的孔洞结构，经壳聚糖/rhBMP-2溶液浸泡后孔洞被填充覆盖。改性后NiTi合金表面亲水角明显降低，在模拟体液中rhBMP2释放的时间逐渐延长。改性后NiTi合金与小鼠成骨细胞共培养后碱性磷酸酶较对照组显著增加。说明改性后的合金亲水性显著改善，在模拟体液环境中rhBMP-2能够缓慢释放，具有促进小鼠成骨细胞增殖的能力。 |
| 关键词: NiTi合金 骨形态蛋白 缓释 |
| DOI： |
| 分类号: |
| 基金项目:甘肃省卫生行业计划（GSWST2011072）； 甘肃省自然科学基金（1208KJZA273） |
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| Preparation and Properties in Vitro of RhBMP-2 Loaded Chitosan Microsphere Coated by Nitinol |
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XU Li-hu, YUN Xiang-dong, CHEN Yuan-zhe, AN Li-ping, XIA Ya-yi1,2
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1a. Institute of Orthopaedics, 1b. Orthopaedics Key Laboratory of Gansu Province, Lanzhou University Second Hospital, Lanzhou 730030;2. The Institute of Chemical Technology, Lanzhou University, Lanzhou 730030
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| Abstract: |
| Chitosan/rhBMP-2 was coated on the nitinol alloy surface that had been fabricated with PAA/PAH through self-assembly and dissolution methods. The alloy surface structure was observed under the scanning electronic microscope after every fabricated procedure. The contact-angle was measured to characterize the nitinol alloy surface hydrophilicity. The protein release rate was detected by enzyme-linked immunosorbent assays after alloy steeped into simulated body fluid. Alkaline phosphatase (ALP) activity was detected to evaluate the effect of alloy on mouse osteoblast growth. Results showed that after being coated with the solution of chitosan/rhBMP-2, hydrophilic character of the alloy surface increases. With the increase of the soaking time, rhBMP-2 gradually increases, and the activity of ALP in cell-culture medium obviously increases. A nitinol alloy with new surface characteristic was successfully made. |
| Key words: nitinol alloy bone morphogenetic protein (BMP) sustained-release |
